General

Cell Line
hPSCreg name	YSCHi002-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	YSCHi002-A (RRID:CVCL_D0QD)
Alternative name(s)	2ABWS
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	YSCHi001-A (2AMJH) Donor diseases: epilepsy SHCDNi006-A (SHCDN006) Donor diseases: epilepsy PFIZi025-A (B220c16) Donor's gene variants: KCNC1 Donor diseases: epilepsy INSRMi004-A (PC118_c7) Donor's gene variants: SCN1A Donor diseases: epilepsy INSRMi006-A (PC131_c8) Donor's gene variants: DEPDC5 Donor diseases: epilepsy INSRMi002-A (PC117_c2) Donor's gene variants: DEPDC5 Donor diseases: epilepsy PFIZi024-A (B218c6) Donor's gene variants: GABRA1 Donor diseases: epilepsy
Last update	18th May 2023
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Provider
Generator	Severance Children’s Hospital (YSCH)
External Databases
BioSamples	SAMEA113484744
Cellosaurus	CVCL_D0QD
Wikidata	Q123033963
General Information
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Epilepsy Synonyms epilepsy Epileptic attack, NOS Epilepsy, unspecified, with intractable epilepsy Generalized seizure (finding) Epileptic seizures Other forms of epilepsy (disorder) Epileptic seizures, NOS [X]Other epilepsy (disorder) Seizure disorder (disorder) Generalized fit Epilepsy (disorder) Epileptic fits Seizure disorder Other forms of epilepsy Epilectic attack, NOS Other forms of epilepsy, with intractable epilepsy Epileptic fits, NOS Epileptic disorder Epilepsy, NOS EPILEP NEC W/O INTR EPIL EPILEPSY NOS W INTR EPIL Epileptic Seizure Epileptic attack Epileptic seizure (finding) seizure disorder [X]Other epilepsy EP - Epilepsy EPILEPSY NEC W INTR EPIL Other forms of epilepsy NOS Other forms of epilepsy and recurrent seizures Epileptic convulsions, NOS Epileptic disorder, NOS Epilepsy, unspecified, without mention of intractable epilepsy Epilepsy, unspecified Epilepsy and recurrent seizures Epilepsy NOS (disorder) EPILEP NOS W/O INTR EPIL Epileptic fit Epilepsy NOS Other forms of epilepsy, without mention of intractable epilepsy Epileptic Generalized seizure Generalized convulsion Generalised seizure Generalised convulsion Other forms of epilepsy NOS (disorder) EF - Epileptic fit Generalised fit Epileptic convulsions show more synonyms show less synonyms
External Databases (Donor)
BioSamples	SAMEA113484756

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	Yes
How may genetic information associated with the cell line be accessed?	Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	세브란스병원 임상연구보호센터
Approval number	4-2018-0021
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Monocyte Large, phagocytic mononuclear leukocytes produced in the vertebrate bone marrow and released into the blood; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles. Synonyms Monocytes MONOCYTE Monocyte monocyte show more synonyms show less synonyms
Reprogramming method
Vector type	Integrating
Vector	Plasmid
Genes	POU5F1 SOX2 KLF4 MYC
Is the used vector excisable?	Unknown
Absence of reprogramming vector(s)?	Unknown
Reprogramming vectors silenced?
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Medium

mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes	–

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Morphology

DAPI-a-muscleactin-2.tif

Endoderm Maker - muscleactin

DAPI-BRY-2.tif

Endoderm Maker - BRY

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Morphology

BWS-AFP-2.tif

Mesoderm Maker - AFP

BWS-FOXA2-2.tif

Mesoderm Maker - FOXA2

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Morphology

BWS-PAX6-3.tif

Ectoderm Maker - PAX6

BWS-TUJ1-3.tif

Ectoderm Maker - TUJ1

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes G-BANDING 1A2eipsc_9K_염색체분석사진.jpg
Other Genotyping (Cell Line)