SA348
CEBe023-A
General
Cell Line |
|
| hPSCreg name | CEBe023-A |
| Cite as: | CEBe023-A (RRID:CVCL_B308) |
| Alternative name(s) |
SA348
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 31st August 2022 |
| User feedback | |
Provider |
|
| Generator |
Takara Bio Europe AB (former Cellartis) (CEB)
Contact:
Human ES cell line derivation |
| Derivation country | Sweden |
External Databases |
|
| Cellosaurus | CVCL_B308 |
| Wikidata | Q54952029 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Date of derivation | 2003-08-26 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | None |
| Cell seeding | Embryo |
Culture Conditions
| Feeder cells |
ES-C57BL/6 Cellfinder Ont Id: CLO_0002948 |
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| Medium |
VitroHES
Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| hES-Cellect |
Yes |
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| SSEA-1 |
No |
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| hTERT |
Yes |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 51
|
Other Genotyping (Cell Line) |
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