SA348

General

Cell Line

hPSCreg Name
CEBe023-A
Alternative name(s)
SA348
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Takara Bio Europe AB (former Cellartis) (CEB)
Derivation country Sweden

External Databases

Cellosaurus CVCL_B308
CLO CLO_0100059
Wikidata Q54952029

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation

Date of derivation 2003-08-26
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation None
Cell seeding Embryo

Culture Conditions

Feeder cells ES-C57BL/6
Cellfinder Ont Id: CLO_0002948
Medium VitroHES
Supplements
Serum Replacement %
hrbFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
hES-Cellect
Yes
SSEA-1
No
hTERT
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 51

Other Genotyping (Cell Line)