CCTL 9

General

Cell Line

hPSCreg name MUNIe003-A
Cite as:
MUNIe003-A (RRID:CVCL_C863)
Alternative name(s)
CCTL 9
Cell line type Human embryonic stem cell (hESC)
Similar lines
Last update 30th August 2022
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Provider

Generator Masaryk University (MUNI)
Derivation country Czech Republic

External Databases

Cellosaurus CVCL_C863
Wikidata Q54907080

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Subclones

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethical Comitee Institute of Experimental Medicine, Academy of Science,Czech Republic/Ministry of Education, Youth and Sports
Approval number

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Embryo

Culture Conditions

Surface coating MEF
Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source:
Serum concentration: %
Supplements
FGF2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 58

Other Genotyping (Cell Line)