Generation and characterization of a human iPSC line derived from congenital clubfoot amniotic fluid cells
Summary
A human induced pluripotent stem cell (iPSC) line (SMBCi002-A) was established from amniocytes (amniotic fluid cells, AFDCs) from a 16-week-old female fetus with clubfoot. Reprogramming and enhancing factors OCT4, SOX2, KLF4, MYC, LIN28 and EBNA1 were delivered using a nonintegrative strategy based on episomal plasmids. Validation assays indicated that this iPSC line has full pluripotency, differentiation potential and genetic stability. Currently, the etiology and mechanism of clubfoot remain unclear; therefore, this in vitro cellular model would provide a useful resource for understanding the pathology of this disease. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wang J, Cui Y, Xing K, Luan J, Han J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Mar;43:101712 |
| PubMed | 31986484 |
| DOI | 10.1016/j.scr.2020.101712 |