Generation and characterization of a human iPSC line derived from congenital clubfoot amniotic fluid cells


A human induced pluripotent stem cell (iPSC) line (SMBCi002-A) was established from amniocytes (amniotic fluid cells, AFDCs) from a 16-week-old female fetus with clubfoot. Reprogramming and enhancing factors OCT4, SOX2, KLF4, MYC, LIN28 and EBNA1 were delivered using a nonintegrative strategy based on episomal plasmids. Validation assays indicated that this iPSC line has full pluripotency, differentiation potential and genetic stability. Currently, the etiology and mechanism of clubfoot remain unclear; therefore, this in vitro cellular model would provide a useful resource for understanding the pathology of this disease. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wang J, Cui Y, Xing K, Luan J, Han J
Journal Stem cell research
Publication Date 2020 Mar;43:101712
PubMed 31986484
DOI 10.1016/j.scr.2020.101712

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