human amniotic fluid stem cells derived iPSC, hAFSC-iPSC
NCKUi001-A
General
Cell Line |
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| hPSCreg name | NCKUi001-A |
| Cite as: | NCKUi001-A (RRID:CVCL_C9BQ) |
| Alternative name(s) |
human amniotic fluid stem cells derived iPSC, hAFSC-iPSC
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 31st August 2023 |
| User feedback | |
Provider |
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| Generator | National Cheng Kung University (NCKU) |
External Databases |
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| BioSamples | SAMEA114312890 |
| Cellosaurus | CVCL_C9BQ |
| Wikidata | Q123033161 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
This cell line is the property of Professor Yan-Wen Liu at the Cell Therapy Center of National Cheng Kung University, Tainan, Taiwan. Researchers interested in utilizing this cell line for their studies are requested to apply to Professor Liu's laboratory. Email: wen036030@gmail.com |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA114312889 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | wen036030@gmail.com; cyj@firdi.org.tw |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | Dr. Shiaw-Min Hwang; Dr. Yu-Jen Chang, Bioresource Collection and Research Center, Hsinchu, Taiwan |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type | |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Sendai virus) |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Other medium:
Base medium: StemFlex
Main protein source: StemFlex Supplement medium Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| SSEA-4 |
Yes |
Differentiation Potency
In vivo teratoma
Morphology
In vivo teratoma
Morphology
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Unknown
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Other Genotyping (Cell Line) |
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