INSRMe012-A

STR-I-231-MTMX

General

Cell Line

hPSCreg Name
INSRMe012-A
Alternative name(s)
STR-I-231-MTMX
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator INSERM (INSRM)
Derivation country France

External Databases

Cellosaurus CVCL_C090
CLO CLO_0102628
Wikidata Q54970624

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
X-linked myopathy with excessive autophagy
The donor is affected.
Disease associated phenotypes
  • MYOTUBULAR MYOPATHY X linked

Ethics

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
Yes
ZP removal technique Enzymatic

Culture Conditions

Surface coating Gelatin
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 12

Other Genotyping (Cell Line)