LBCn3

General#

Cell Line

hPSCreg Name EDi021-A
Alternative name(s)
LBCn3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th January 2019
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Provider

Generator University of Edinburgh (ED)

External Databases

BioSamples SAMEA5447460

General Information

Projects
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male
Ethnicity White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
normal Karyotype by G-Banding
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5274196

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection Absence of plasmid integration confirmed by gDNA PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Collagenase and Dispase
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Marker Present Absent
mCpG
OCT4 X
Self-renewal
Positive
Endoderm
Unkown
Mesoderm
Unkown
Ectoderm score
Unkown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal male karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)