Generation and characterisation of a human induced pluripotent stem cell line, NIMHi007-A, from peripheral blood mononuclear cells derived from an adult healthy female
Summary
We report the generation and characterisation of a human induced pluripotent stem cell (iPSC) line, NIMHi007-A, derived from peripheral blood mononuclear cells (PBMCs) of a healthy female adult individual. PBMCs were reprogrammed using the non-integrating Sendai virus consisting of Yamanaka reprogramming factors- SOX2, cMYC, KLF4, and OCT4. The iPSCs displayed a normal karyotype, express pluripotency markers, and could generate into three germ layers, endoderm, mesoderm, and ectoderm, in-vitro. This iPSC line, NIMHi007-A, can be used as a healthy control for various in-vitro disease models and study their underlying pathophysiological mechanisms. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Nimonkar MM, Arunachal G, Dash SS, Kandy SK, Raghavendra K, Chetan GK, Mehta B, Markandeya YS |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Aug;70:103130 |
| PubMed | 37269666 |
| DOI | 10.1016/j.scr.2023.103130 |