Generation of an induced pluripotent stem cell line from a Huntington's disease patient with a long HTT-PolyQ sequence


Huntington's disease (HD) is an inherited neurodegenerative disorder caused by an abnormal length of CAG repeats in the gene HTT, leading to an elongated poly-glutamine (poly-Q) sequence in huntingtin (HTT). We used non-integrative Sendai virus to reprogram fibroblasts from a patient with juvenile onset HD to induced pluripotent stem cells (iPSCs). Reprogrammed iPSCs expressed pluripotency-associated markers, exhibited a normal karyotype, and following directed differentiation generated cell types belonging to the three germ layers. PCR analysis and sequencing confirmed the HD patient-derived iPSC line had one normal HTT allele and one with elongated CAG repeats, equivalent to ≥180Q. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Miller DC, Lisowski P, Genehr C, Wanker EE, Priller J, Prigione A, Diecke S
Journal Stem cell research
Publication Date 2023 Apr;68:103056
PubMed 36863131
DOI 10.1016/j.scr.2023.103056

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