3026-iso, GBA N370S-correction/3026
CBIGi003-A-1
General#
Cell Line |
|
| hPSCreg Name | CBIGi003-A-1 |
| Alternative name(s) |
3026-iso, GBA N370S-correction/3026
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th March 2022 |
| Notes | Isogenic control for CBIGi003-A. |
| User feedback | |
Provider |
|
| Generator | Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG) |
External Databases |
|
| BioSamples | SAMEA13204589 |
| CLO | CLO_0103796 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
|
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA13204588 |
Ethics#
Also have a look at the ethics information for the parental line
CBIGi003-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation#
General |
|
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The source cell information can be found in the parental cell line
CBIGi003-A.
|
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XX
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Genetic modifications not related to a disease |
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