3026-iso, GBA N370S-correction/3026
CBIGi003-A-1
General#
Cell Line |
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hPSCreg Name | CBIGi003-A-1 |
Alternative name(s) |
3026-iso, GBA N370S-correction/3026
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th March 2022 |
Notes | Isogenic control for CBIGi003-A. |
User feedback | |
Provider |
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Generator | Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG) |
External Databases |
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BioSamples | SAMEA13204589 |
CLO | CLO_0103796 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
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Subclone of |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA13204588 |
Ethics#
Also have a look at the ethics information for the parental line
CBIGi003-A
.
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
CBIGi003-A.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Is reprogramming vector detectable? |
No |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
Genetic Modification#
Genetic modifications not related to a disease |
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