RUES1
RUESe001-A
General#
Cell Line |
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| hPSCreg Name | RUESe001-A |
| Alternative name(s) |
RUES1
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 22nd October 2017 |
| User feedback | |
Provider |
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| Generator |
The Rockefeller University (RUES)
Contact:
Laboratory of Molecular Embryology |
| Owner | Wyss Institute for Biologically Inspired Engineering at Harvard University |
| Distributors | |
| Derivation country | United States |
External Databases |
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| Cellosaurus | CVCL_B809 |
| BioSamples | SAMEA104387754 |
| NIHhESC | NIHhESC-09-0012 |
General Information |
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| Publications |
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| Projects | |
| Is the cell line available in principle? | Available with restrictions: Available for clinical and commercial research |
Donor Information#
General Donor Information |
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| Sex | female |
| Ethnicity | unknown |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Family history | NA |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Ethics#
We are currently revising this section.hESC Derivation#
| Date of derivation | 2004-08-30 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
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| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
Culture Conditions#
| Feeder cells |
MEF Cellfinder Ont Id: EFO_0004040 |
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| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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