RUES1
RUESe001-A
General
Cell Line |
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hPSCreg Name | RUESe001-A |
Alternative name(s) |
RUES1
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 26th January 2023 |
User feedback | |
Provider |
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Generator |
The Rockefeller University (RUES)
Contact:
Laboratory of Molecular Embryology |
Owner | Wyss Institute for Biologically Inspired Engineering at Harvard University |
Distributors | |
Derivation country | United States |
External Databases |
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Cellosaurus | CVCL_B809 |
BioSamples | SAMEA104387754 |
NIHhESC | NIHhESC-09-0012 |
CLO | CLO_0101496 |
Wikidata | Q54951467 |
General Information |
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Publications |
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Projects | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information
General Donor Information |
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Sex | female |
Ethnicity | unknown |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Disease associated phenotypes | no phenotypes |
Family history | NA |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
Yes
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External Databases (Donor) |
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BioSamples | SAMEA8621007 |
Ethics
Was the embryo established purely for research purposes? | No |
Have both parents consented to the use of the embryo for ESC derivation? | Yes |
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
If you do not hold the Donor Consent Form, do you know who does? | No |
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | NIHhESC-09-0012 |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent expressly prevent the derivation of pluripotent stem cells? | No |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | No information |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Clinical Research and Development Services Corp. |
Approval number | 03-06-01 |
hESC Derivation
Date of derivation | 2004-08-30 |
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
No |
ZP removal technique | Enzymatic |
Cell isolation | Immunosurgery |
Culture Conditions
Feeder cells |
MEF Cellfinder Ont Id: EFO_0004040 |
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Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Alkaline Phosphatase |
Yes |
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SSEA-3 |
Yes |
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TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 15
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
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