RUES1

General#

Cell Line

hPSCreg Name RUESe001-A
Alternative name(s)
RUES1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd October 2017
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Provider

Generator The Rockefeller University (RUES)
Owner Wyss Institute for Biologically Inspired Engineering at Harvard University
Distributors
Derivation country United States

External Databases

Cellosaurus CVCL_B809
BioSamples SAMEA104387754
NIHhESC NIHhESC-09-0012

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for clinical and commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history NA
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2004-08-30
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery

Culture Conditions#

Feeder cells MEF
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
SSEA-3
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes