NCRM-5, NL-5


Cell Line

hPSCreg name CRMi001-A
Cite as:
CRMi001-A (RRID:CVCL_1E75)
Alternative name(s)
NCRM-5, NL-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Generator National Institutes of Health - Center for Regenerative Medicine (CRM)
Owner National Institutes of Health - Center for Regenerative Medicine (CRM)
Derivation country United States

External Databases

BioSamples SAMEA7775668
Cellosaurus CVCL_1E75
Wikidata Q54908259

General Information

* Is the cell line readily obtainable for third parties?
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) fetal

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes
  • Control

External Databases (Donor)

BioSamples SAMEA7774918


Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? Yes
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Name of accrediting authority involved? Redacted form available from distributor
Approval number
Name of accrediting authority involved? Redacted form available from distributor
Approval number
Please describe:
Further constraints on use
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation


Source cell origin
Blood that remains in the placenta and in the attached umbilical cord after childbirth[WP].; Cord blood is collected because it contains stem cells, which can be used to treat hematopoietic and genetic disorders.[WP]
  • cord blood
  • fetal blood
  • umbilical cord blood
Source cell type (free text) Cord Blood CD34+ cells
Age of donor (at collection) fetal

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming


Selection criteria for clones Colony morphology and alkaline phosphatase expression
Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
Passage method Enzymatically
O2 Concentration 4 %
CO2 Concentration 5 %
Medium mTeSR™ 1


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
POU5F1 (OCT-4)
TRA 1-60
Differentiation Potency
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Passage: 12


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
46 X,Y
Passage number: 8
Karyotyping method: G-Banding

Other Genotyping (Cell Line)