NCRM-5, NL-5

General#

Cell Line

hPSCreg Name CRMi001-A
Alternative name(s)
NCRM-5, NL-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th September 2018
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Provider

Generator National Institutes of Health - Center for Regenerative Medicine (CRM)
Owner National Institutes of Health - Center for Regenerative Medicine (CRM)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA7775668
Cellosaurus CVCL_1E75

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for commercial research
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes
  • Control

External Databases (Donor)

BioSamples SAMEA7774918

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? Yes
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Policy for access to genetic information Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Name of accrediting authority involved? Redacted form available from distributor
Approval number
Name of accrediting authority involved? Redacted form available from distributor
Approval number
Please describe:
Further constraints on use
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation#

General

Source cell origin
umbilical cord blood
blood that remains in the placenta and in the attached umbilical cord after childbirth[WP].
Source cell type (free text) Cord Blood CD34+ cells
Age of donor (at collection) fetal

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Selection criteria for clones Colony morphology and alkaline phosphatase expression
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
O2 Concentration 4 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Unkown
POU5F1 (OCT-4)
Yes
SSEA-3
Unkown
SSEA-4
Unkown
TRA 1-60
Yes
TRA 1-81
Unkown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 12

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 X,Y
Karyotyping method: G-Banding

Other Genotyping (Cell Line)