NCRM-5, NL-5

General#

Cell Line

hPSCreg Name CRMi001-A
Alternative name(s)
NCRM-5, NL-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th September 2018
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Provider

Generator National Institutes of Health - Center for Regenerative Medicine (CRM)
Owner National Institutes of Health - Center for Regenerative Medicine (CRM)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA7775668
Cellosaurus CVCL_1E75

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for commercial research
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes
  • Control

External Databases (Donor)

BioSamples SAMEA7774918

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell origin
umbilical cord blood
blood that remains in the placenta and in the attached umbilical cord after childbirth[WP].
Source cell type (free text) Cord Blood CD34+ cells
Age of donor (at collection) fetal

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Selection criteria for clones Colony morphology and alkaline phosphatase expression
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
O2 Concentration 4 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Unkown
POU5F1 (OCT-4)
Yes
SSEA-3
Unkown
SSEA-4
Unkown
TRA 1-60
Yes
TRA 1-81
Unkown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 12

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 X,Y
Karyotyping method: G-Banding

Other Genotyping (Cell Line)