Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay
Summary
To understand how potential gene manipulations affect in vitro microglia, we provide a set of short protocols to evaluate microglia identity and function. We detail steps for immunostaining to determine microglia identity. We describe three functional assays for microglia: phagocytosis, calcium response following ATP stimulation, and cytokine expression upon inflammatory stimuli. We apply these protocols to human induced-pluripotent-stem-cell (hiPSC)-derived microglia, but they can be also applied to other in vitro microglial models including primary mouse microglia. For complete details on the use and execution of this protocol, please refer to Bartalska et al. (2022).1. Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
| Authors | Hübschmann V, Korkut-Demirbaş M, Siegert S |
|---|---|
| Journal | STAR protocols |
| Publication Date | 2022 Dec 16;3(4):101866 |
| PubMed | 36595902 |
| PubMed Central | PMC9678782 |
| DOI | 10.1016/j.xpro.2022.101866 |