SCVI 2176
SCVIi123-A
General
Cell Line |
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| hPSCreg name | SCVIi123-A |
| Cite as: | SCVIi123-A |
| Alternative name(s) |
SCVI 2176
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 14th May 2024 |
| User feedback | |
Provider |
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| Generator | Stanford Cardiovascular Institute (SCVI) |
External Databases |
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| BioSamples | SAMEA115580282 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | White |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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External Databases (Donor) |
|
| BioSamples | SAMEA115580283 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | Dr Joseph C WU |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | Dr Karim Sallam |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | The generation of the lines was approved by the Administrative Panel on Human Subjects Research (IRB) under IRB #29904 “Derivation of Human Induced Pluripotent Stem Cells (Biorepository)”. |
| Approval number | IRB #29904 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell line name |
PBMC Derived from same source line (potentially other lot and donor, see below):
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| Source cell type |
An immature, nucleated erythrocyte occupying the stage of erythropoiesis that follows formation of erythroid progenitor cells and precedes formation of reticulocytes.
Synonyms
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| Source cell origin |
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
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| Source cell type (free text) | Arm Venipuncture |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| SOX17 – SRY-box transcription factor 17 |
Yes |
| FOXA2 – forkhead box A2 |
Yes |
Protocol or reference
Screenshot 2024-05-12 at 8.14.11 AM.png
DAPI(Blue), SOX17 (GREEN) and FOXA2(Red)
In vitro spontaneous differentiation
| Marker | Expressed |
| TBX6 – T-box transcription factor 6 |
Yes |
Protocol or reference
Screenshot 2024-05-12 at 8.15.02 AM.png
DAPI(Blue), BRACHYUN (GREEN) and TBX6(Red)
In vitro spontaneous differentiation
| Marker | Expressed |
| OTX2 – orthodenticle homeobox 2 |
Yes |
| PAX6 – paired box 6 |
Yes |
Protocol or reference
Screenshot 2024-05-13 at 5.04.29 AM.png
DAPI(Blue), OTX2 (GREEN) and PAX6(Red)
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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