High-content screening of mitochondrial polarization in neural cells derived from human pluripotent stem cells


We present a high-content screening (HCS) protocol for quantifying mitochondrial activity in live neural cells from human induced pluripotent stem cells (iPSCs). The assessment is based on mitochondrial membrane potential, which is influenced by the efficiency of mitochondrial bioenergetics. We describe how to perform the analysis using both an HCS platform and the open-source software CellProfiler. The protocol can identify the mitochondrial fitness of human neurons and may be used to carry out high-throughput compound screenings in patient-derived neural cells. For complete details on the use and execution of this protocol, please refer to Lorenz et al. (2017) and Zink et al. (2020). © 2022 The Author(s).

Authors Zink A, Haferkamp U, Wittich A, Beller M, Pless O, Prigione A
Journal STAR protocols
Publication Date 2022 Sep 16;3(3):101602
PubMed 35959496
PubMed Central PMC9361325
DOI 10.1016/j.xpro.2022.101602

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