I6

General#

Cell Line

hPSCreg Name TECHe003-A
Alternative name(s)
I6
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Technion - Israel Institute of Technology (IIT)
Derivation country Israel

External Databases

Cellosaurus CVCL_C380
NIHhESC NIHhESC-13-0206

General Information

Publications
Is the cell line available in principle?
Yes
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
No
SOX2
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)