CLS2
AAUe002-A
General
Cell Line |
|
| hPSCreg name | AAUe002-A |
| Cite as: | AAUe002-A (RRID:CVCL_C741) |
| Alternative name(s) |
CLS2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator |
Aalborg University (AAU)
Contact:
Laboratoriet for Stamcelleforskning |
| Derivation country | Denmark |
External Databases |
|
| Cellosaurus | CVCL_C741 |
| Wikidata | Q54813676 |
General Information |
|
| Publications |
|
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| Feeder cells |
human foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
|||||||||
| Passage method | Mechanically | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| Telomerase Activity |
Yes |
|
||||
| FGF4 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 20
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.