A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells

Summary

The current procedure thoroughly explains how to reprogram induced pluripotent stem cells (iPSCs) from the patient's peripheral blood mononuclear cells (PBMCs), a less invasive source; e.g., somatic cells. We describe how to isolate PBMCs and reprogram them into iPSCs by electroporation. Furthermore, we provide an alternative approach to generating iPSC using Geltrex or Matrigel matrix to replace MEF-feeder. The challenge of this process is the relatively lower cell survival rates of PBMCs due to the damage of electroporation. For complete details on the use and execution of this protocol, please refer to Hu et al. (2021). Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Authors Gao L, Wang F, Wang Y, Hu L, Mao J
Journal STAR protocols
Publication Date 2022 Jul 12;3(3):101530
PubMed 35834385
PubMed Central PMC9287807
DOI 10.1016/j.xpro.2022.101530

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