Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female


Human lymphoblast cells were used to generate integration-free induced pluripotent stem cells (iPSCs) employing episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. The derived iPSCs were defined as pluripotent based on (i) expression of pluripotency-associated markers, (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptomes of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0.95. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Martins S, Bohndorf M, Schröter F, Assar F, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Journal Stem cell research
Publication Date 2018 Jan;26:76-79
PubMed 29268155
DOI 10.1016/j.scr.2017.12.007

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