Generation of a heterozygous SOX10 knockout human embryonic stem cell line using CRISPR/Cas9 technology
SOX10 is one of the master transcription factors in neural crest development. Human SOX10 mutations are associated with Waardenburg syndrome type 4 (Waardenburg-Shah, WS4), which can be inherited in both autosomal dominant and recessive patterns. Here, the human embryonic stem cell (hESC) line, H9, was used to generate a heterozygous SOX10 knockout cell line as the in vitro model of WS4 by CRISPR/Cas9-mediated gene targeting. This cell line may represent a valuable tool for uncovering the pathogenesis of WS4. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Wang T, Zhang J, Liu J, Lai X|
|Journal||Stem cell research|
|Publication Date||2021 Oct 11;57:102567|