Generation of a GPR146 knockout human induced pluripotent stem cell line (ITXi001-A-1)


Dyslipidemia is a key modifiable causal risk factor involved in the development of atherosclerotic cardiovascular disease. Recently, the G protein-coupled receptor 146 (GPR146), a member of the G-coupled protein receptors' family, has been shown to be a regulator of plasma cholesterol. Inhibition of hepatic GPR146 in mice displays protective effect against both hypercholesterolemia and atherosclerosis. Here, we characterize a genetically engineered human induced pluripotent stem cell (hiPSC) model invalidated for GPR146 (ITXi001-A-1) using CRISPR-Cas9 editing technology. Differentiation of ITXi001-A-1 towards hepatic fate will provide a suitable model for deciphering the molecular mechanisms sustaining the beneficial metabolic effects of GPR146 inhibition. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Bray L, Caillaud A, Girardeau A, Patitucci M, Le May C, Cariou B, Rimbert A
Journal Stem cell research
Publication Date 2022 Apr;60:102721
PubMed 35247835
DOI 10.1016/j.scr.2022.102721

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