ITXi001-A-1

GPR146KO Cl2

General

Cell Line

hPSCreg Name
ITXi001-A-1
Alternative name(s)
GPR146KO Cl2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th February 2022
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Provider

Generator l’institut du thorax (ITX)
Owner l’institut du thorax (ITX)
Derivation country France

External Databases

BioSamples SAMEA10368000
CLO CLO_0103774

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasienne

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA10367957

Ethics

Also have a look at the ethics information for the parental line ITXi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line ITXi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: iPS Brew
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
NANOG
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
HAND1
Yes
T(TBXT)
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
SOX1
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Gene knock-out
g.chr7:1,057,542-1,057,944del.
Deletion of 402pb : (GRCh38/hg38): g.chr7:1,057,542-1,057,944del. (NM_138445.4):c.164-566del. (NP_612454.1):p.Thr10ArgfsX12.
CRISPR-associated (CRISPR/Cas) System