Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male
Summary
We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming. Copyright © 2021. Published by Elsevier B.V.
| Authors | Ge N, Liu M, Zhu X, Krawczyk J, McInerney V, Shen S, O'Brien T, Prendiville T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102389 |
| PubMed | 34088016 |
| DOI | 10.1016/j.scr.2021.102389 |