hPSCreg®
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NCCSEDi001-A

Registration Summary:
NCCSEDi001-A

General

Cell Line
hPSCreg name NCCSEDi001-A
Cite as:
NCCSEDi001-A (RRID:CVCL_A4EF)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 28th July 2020
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Provider
Generator National Chengdu Center for Safety Evaluation of Drugs (NCCSED)
Derivation country China

External Databases
BioSamples SAMEA7200924
Cellosaurus CVCL_A4EF
Wikidata Q105510304

General Information
Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information
Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

Karyotyping (Donor)
Has the donor karyotype been analysed?
Unknown

External Databases (Donor)
BioSamples SAMEA7200925

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: buqian7978@scu.edu.cn
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink buqian7978@scu.edu.cn
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of West China Hospital of Sichuan University
Approval number 2017（169）
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of West China Hospital of Sichuan University
Approval number 2017（169）
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type
Fibroblast
A connective-tissue cell of mesenchymal origin that secretes proteins and especially molecular collagen from which the extracellular fibrillar matrix of connective tissue forms.

Reprogramming method
Vector type None

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46，XY
3H.tif

Other Genotyping (Cell Line)