Generation of an NANS homozygous knockout human induced pluripotent stem cell line by the insertion of GFP-P2A-Puro via CRISPR/Cas9 editing


N-acetylneuraminic acid synthase (NANS), the gene encoding the synthase for N-acetylneuraminic acid (NeuNAc; sialic acid), is closely associated with infantile-onset severe developmental delay and skeletal dysplasia. However, the role and the involved mechanisms of NANS functioning have not been fully understood to date. Here, we generated a homozygous NANS-knockout human induced pluripotent stem cell (iPSC) line, NCCSEDi001-A-1, via the CRISPR/Cas9-based gene editing method. The NCCSEDi001-A-1 cell line does not express NANS protein, but maintains a normal karyotype, pluripotency, and trilineage differentiation potential. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Bu Q, Zhang H, Liu Q, Dai Y, Wei Q, Xue A, Huang Y, Zhong K, Huang Y, Gao H, Cen X
Journal Stem cell research
Publication Date 2020 Dec;49:102052
PubMed 33130469
DOI 10.1016/j.scr.2020.102052

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