Generation of an NANS homozygous knockout human induced pluripotent stem cell line by the insertion of GFP-P2A-Puro via CRISPR/Cas9 editing
N-acetylneuraminic acid synthase (NANS), the gene encoding the synthase for N-acetylneuraminic acid (NeuNAc; sialic acid), is closely associated with infantile-onset severe developmental delay and skeletal dysplasia. However, the role and the involved mechanisms of NANS functioning have not been fully understood to date. Here, we generated a homozygous NANS-knockout human induced pluripotent stem cell (iPSC) line, NCCSEDi001-A-1, via the CRISPR/Cas9-based gene editing method. The NCCSEDi001-A-1 cell line does not express NANS protein, but maintains a normal karyotype, pluripotency, and trilineage differentiation potential. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Bu Q, Zhang H, Liu Q, Dai Y, Wei Q, Xue A, Huang Y, Zhong K, Huang Y, Gao H, Cen X|
|Journal||Stem cell research|
|Publication Date||2020 Dec;49:102052|