hPSCreg®
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NCCSEDi001-A-1

Registration Summary:
NCCSEDi001-A-1

General

Cell Line
hPSCreg name NCCSEDi001-A-1
Cite as:
NCCSEDi001-A-1 (RRID:CVCL_A4EG)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 21st October 2020
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Provider
Generator National Chengdu Center for Safety Evaluation of Drugs (NCCSED)

External Databases
BioSamples SAMEA7222556
Cellosaurus CVCL_A4EG
Wikidata Q105510307

General Information
Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information
Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

External Databases (Donor)
BioSamples SAMEA7200925

Ethics

Also have a look at the ethics information for the parental line NCCSEDi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line NCCSEDi001-A.

Reprogramming method
Vector type Integrating
Vector Plasmid
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
SOX1
Yes
PAX6
Yes
Morphology
nans-ko-ecto-1.tif

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XY
NCCSEDi001-A-1.tif

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Intellectual developmental disorder 61
Synonyms
  • INTELLECTUAL DEVELOPMENTAL DISORDER 61
  • MRD61
  • Mental Retardation, Autosomal Dominant 61
Genetic modifications
NANS (target)
Gene knock-out
9q22.33
The gene NANS, is located at 9q22.33, also known as sialic acid (phosphate) synthase, encodes N-acetylneuraminic acid 9-phosphate synthase (NeuNAc-9-P synthase), a cytosolic protein approximately 40 kDa in size and comprising 359 amino acids. NeuNAc-9-P synthase plays a pivotal role in the primary synthesis of the most common sialic acid, N-acetylneuraminic acid (Neu5Ac). Furthermore, as a critical step along the pathway towards the production of the principal sialylation substrates, CMP-Neu5Ac and CMP-Neu5Gc, NANS can also influence the sialylation of glycans in important ways. It can mediate or regulate a variety of physiological and pathological processes, such as the correct establishment of the vertebrate embryonic nervous system, inflammation and immune response pathways, and tumorigenesis and metastasis in certain cancers.
CRISPR-associated (CRISPR/Cas) System