NCCSEDi001-A-1

General#

Cell Line

hPSCreg Name NCCSEDi001-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th July 2020
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator National Chengdu Center for Safety Evaluation of Drugs (NCCSED)

External Databases

BioSamples SAMEA7222556
CLO CLO_0102951

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA7200925

Ethics#

Also have a look at the ethics information for the parental line NCCSEDi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line NCCSEDi001-A.

Reprogramming method

Vector type Integrating
Vector Plasmid
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Differentiation Potency
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
SOX1
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
intellectual developmental disorder 61
Genetic modifications
NANS (target)
Gene knock-out
9q22.33
The gene NANS, is located at 9q22.33, also known as sialic acid (phosphate) synthase, encodes N-acetylneuraminic acid 9-phosphate synthase (NeuNAc-9-P synthase), a cytosolic protein approximately 40 kDa in size and comprising 359 amino acids. NeuNAc-9-P synthase plays a pivotal role in the primary synthesis of the most common sialic acid, N-acetylneuraminic acid (Neu5Ac). Furthermore, as a critical step along the pathway towards the production of the principal sialylation substrates, CMP-Neu5Ac and CMP-Neu5Gc, NANS can also influence the sialylation of glycans in important ways. It can mediate or regulate a variety of physiological and pathological processes, such as the correct establishment of the vertebrate embryonic nervous system, inflammation and immune response pathways, and tumorigenesis and metastasis in certain cancers.
CRISPR-associated (CRISPR/Cas) System