Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing
Summary
Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Diouf D, Vitale MR, Zöller JEM, Pineau AM, Klopocki E, Hamann C, Ziegler GC, Vanmierlo T, Van den Hove D, Lesch KP |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Mar;67:103038 |
| PubMed | 36746102 |
| DOI | 10.1016/j.scr.2023.103038 |