Generation of multiple human iPSC lines from peripheral blood mononuclear cells of two SLC2A3 deletion and two SLC2A3 duplication carriers


Copy number variants of SLC2A3, which encodes the glucose transporter GLUT3, are associated with several neuropsychiatric and cardiac diseases. Here, we report the successful reprogramming of peripheral blood mononuclear cells from two SLC2A3 duplication and two SLC2A3 deletion carriers and subsequent generation of two transgene-free iPSC clones per donor by Sendai viral transduction. All eight clones represent bona fide hiPSCs with high expression of pluripotency genes, ability to differentiate into cells of all three germ layers and normal karyotype. The generated cell lines will be helpful to enlighten the role of glucometabolic alterations in pathophysiological processes shared across organ boundaries. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Ziegler GC, Radtke F, Vitale MR, Preuße A, Klopocki E, Herms S, Lesch KP
Journal Stem cell research
Publication Date 2021 Oct;56:102526
PubMed 34492570
DOI 10.1016/j.scr.2021.102526

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