Generation of CDMLe012-A-1 cells: A pluripotent human embryonic stem cell model of Turner's syndrome
Summary
Monosomy of chromosome X is associated with high prenatal mortality of female embryos and severe developmental abnormalities of patients born with Turner's syndrome (45,XO). The CDMLe012-A-1 human embryonic stem cell (hESC) line, derived from a day six blastocyst with a normal 46,XX female karyotype spontaneously lost an X-chromosome during cell culture. This 45,XO karyotype was stably maintained for more than 55 passages. Since the CDMLe012-A-1 cells express pluripotent stem cell markers and differentiate into cells derived from the three germ layers, the cell line represents a stable, pluripotent stem cell model of Turner's syndrome. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Domozhirov AY, Mazzilli JL, Wetsel RA, Zsigmond EM |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Aug;39:101508 |
| PubMed | 31357114 |
| DOI | 10.1016/j.scr.2019.101508 |