Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives


Four human iPSC cell lines (one Jervell and Lange-Nielsen Syndrome, one Long QT Syndrome-type 1 and two healthy controls) were generated from peripheral blood obtained from donors belonging to the same family. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing OCT3/4, KLF4, SOX2 and cMYC as reprogramming factors) was used to generate all cell lines. The four iPSCs have normal karyotype, express pluripotency markers as determined by RT-PCR and flow cytometry and differentiated spontaneously in vitro into cells of the three germ layers, confirming their pluripotent capacity. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Kasai-Brunswick TH, Silva Dos Santos D, Ferreira RP, Araujo DS, Dias GM, Coutinho JLA, Cruz FESF, Sternick EB, Gubert F, Oliveira JCG, Vaz IM, Borgonovo T, Brofman PRS, Moura-Neto RS, Silva R, Campos-de-Carvalho AC, Carvalho AB
Journal Stem cell research
Publication Date 2018 Aug;31:174-180
PubMed 30099333
DOI 10.1016/j.scr.2018.07.016

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