Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls


Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Raska J, Klimova H, Sheardova K, Fedorova V, Hribkova H, Pospisilova V, Vochyanova S, Vanova T, Bohaciakova D
Journal Stem cell research
Publication Date 2021 May;53:102378
PubMed 34088007
DOI 10.1016/j.scr.2021.102378

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