Cyth25
SALKe001-A
General
iPSC Line |
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| hPSCreg name | SALKe001-A |
| Cite as: | SALKe001-A (RRID:CVCL_B851) |
| Alternative name(s) |
Cyth25
|
| iPSC line type | Human embryonic stem cell (hESC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
The Salk Institute for Biological Studies (SALK)
Contact:
Laboratory of Genetics |
| Derivation country | United States |
External iPSC Databases |
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| Cellosaurus | CVCL_B851 |
| Wikidata | Q54817113 |
General iPSC Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent |
hESC Derivation
iPSC Culture Conditions
| Feeder cells |
Mouse Embryonic fibroblast Cellfinder Ont Id: EFO_0004040 |
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| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Genotyping
Karyotyping (iPSC Line) |
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Other Genotyping (iPSC Line) |
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