Cyth25

General#

Cell Line

hPSCreg Name SALKe001-A
Alternative name(s)
Cyth25
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator The Salk Institute for Biological Studies (SALK)
Derivation country United States

External Databases

Cellosaurus CVCL_B851
CLO CLO_0100280

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent

hESC Derivation#

Culture Conditions#

Feeder cells Mouse Embryonic fibroblast
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)