General

Cell Line
hPSCreg name	SALKe001-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	SALKe001-A (RRID:CVCL_B851)
Alternative name(s)	Cyth25
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	22nd May 2019
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Provider
Generator	The Salk Institute for Biological Studies (SALK) Contact: Laboratory of Genetics
Derivation country	United States
External Databases
Cellosaurus	CVCL_B851
Wikidata	Q54817113
General Information
Publications	Muotri AR et al. Development of functional human embryonic stem cell-derived neurons in mouse brain. Proceedings of the National Academy of Sciences of the United States of America. 2005 Dec 20;102(51):18644-8.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Phenotype and Disease related information (Donor)
Diseases

Ethics

Alternatives to consent

hESC Derivation

Culture Conditions

Feeder cells	Mouse Embryonic fibroblast Cellfinder Ont Id: EFO_0004040
Medium	Other medium: Base medium: DMEM/F-12 Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit bFGF 20 %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes
SSEA-1	No
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)