Cyth25

General

Cell Line

hPSCreg Name
SALKe001-A
Alternative name(s)
Cyth25
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator The Salk Institute for Biological Studies (SALK)
Derivation country United States

External Databases

Cellosaurus CVCL_B851
CLO CLO_0100280
Wikidata Q54817113

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent

hESC Derivation

Culture Conditions

Feeder cells Mouse Embryonic fibroblast
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 20 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)