Generation of integration free human iPSC line SORMi002-A from neonatal foreskin fibroblasts


Circumcision is a common surgical procedure usually performed on neonates worldwide. Skin removed after circumcision is abundant and often discarded. Neonatal foreskin fibroblasts (NFFs) can be easily isolated from it for application in biomedical research including induced pluripotent stem cell (iPSC) generation. In this study NFFs were reprogrammed to iPSCs using non-integrating episomal reprogramming plasmids. The iPSCs generated express pluripotency markers. They form embryoid bodies and differentiate in vitro into cells of endoderm, mesoderm and ectoderm. They have a normal male karyotype, are genetically identical to the parent fibroblasts and are transgene free. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Shivaraj A, Parveen S
Journal Stem cell research
Publication Date 2019 Oct;40:101534
PubMed 31454679
DOI 10.1016/j.scr.2019.101534

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