LUMC0116iCRB09 ISO-03, LUMC0116iCRB09 heterozygote CRISPR corrected isogenic clone 03, iso03LUMC0116iCRB09
LUMCi054-A-2
General
Cell Line |
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| hPSCreg name | LUMCi054-A-2 |
| Cite as: | LUMCi054-A-2 |
| Alternative name(s) |
LUMC0116iCRB09 ISO-03, LUMC0116iCRB09 heterozygote CRISPR corrected isogenic clone 03, iso03LUMC0116iCRB09
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
LUMCi054-A (CRB1 patient c.3122T>C p.(Met1041Thr) homozygote, LUMC0116iCRB09) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi054-A-1 (LUMC0116iCRB09 ISO-02, LUMC0116iCRB09 homozygote CRISPR corrected isogenic clone 02, iso02LUMC0116iCRB09) Donor's gene variants: CRB1, CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi056-A-1 (LUMC0128iCRB01 heterozygote CRISPR corrected isogenic clone 02, iso02LUMC0128iCRB01) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi055-A (CRB1 patient 117 compound heterozygous 2983G>T p.(Glu995*) c.1892A>G, p.(Tyr631Cys), LUMC0117iCRB01) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi056-A (CRB1 patient 128 compound heterozygous c.2843G>A p.(Cys948Tyr) and c.3122T>C p.(Met1041Thr), LUMC0128iCRB01) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa |
| Last update | 22nd January 2024 |
| User feedback | |
Provider |
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| Generator | Leiden University Medical Center (LUMC) |
| Owner | Leiden University Medical Center (LUMC) |
| Distributors | |
| Derivation country | Netherlands |
External Databases |
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| BioSamples | SAMEA115121927 |
General Information |
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| Publications |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | white |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Is the medical history available upon request? | yes |
| Is clinical information available? | yes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
normal
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
copy number variation analysis
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External Databases (Donor) |
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| BioSamples | SAMEA115120808 |
Ethics
Also have a look at the ethics information for the parental line
LUMCi054-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | LUMC biobank & EU regulations |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LUMCi054-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus () |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
No |
| Methods used |
Immunostaining, PCR, RT-PCR, Sequencing
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
18
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
normal
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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