LUMC0116iCRB09 ISO-02, LUMC0116iCRB09 homozygote CRISPR corrected isogenic clone 02, iso02LUMC0116iCRB09
LUMCi054-A-1
General
Cell Line |
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| hPSCreg name | LUMCi054-A-1 |
| Cite as: | LUMCi054-A-1 |
| Alternative name(s) |
LUMC0116iCRB09 ISO-02, LUMC0116iCRB09 homozygote CRISPR corrected isogenic clone 02, iso02LUMC0116iCRB09
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
LUMCi054-A (CRB1 patient c.3122T>C p.(Met1041Thr) homozygote, LUMC0116iCRB09) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi054-A-2 (LUMC0116iCRB09 ISO-03, LUMC0116iCRB09 heterozygote CRISPR corrected isogenic clone 03, iso03LUMC0116iCRB09) Donor's gene variants: CRB1, CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi055-A (CRB1 patient 117 compound heterozygous 2983G>T p.(Glu995*) c.1892A>G, p.(Tyr631Cys), LUMC0117iCRB01) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi056-A (CRB1 patient 128 compound heterozygous c.2843G>A p.(Cys948Tyr) and c.3122T>C p.(Met1041Thr), LUMC0128iCRB01) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa LUMCi056-A-1 (LUMC0128iCRB01 heterozygote CRISPR corrected isogenic clone 02, iso02LUMC0128iCRB01) Donor's gene variants: CRB1, CRB1 Donor diseases: Retinitis Pigmentosa |
| Last update | 22nd January 2024 |
| User feedback | |
Provider |
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| Generator | Leiden University Medical Center (LUMC) |
External Databases |
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| BioSamples | SAMEA115120809 |
General Information |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | white |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Is the medical history available upon request? | yes |
| Is clinical information available? | yes |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
copy number variation analysis
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External Databases (Donor) |
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| BioSamples | SAMEA115120808 |
Ethics
Also have a look at the ethics information for the parental line
LUMCi054-A
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| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LUMCi054-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Unknown |
| Methods used |
Immunostaining, PCR, RT-PCR, Sequencing
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Medium |
mTeSR™ Plus
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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