HSF12
SUSMe005-A
General
Cell Line |
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| hPSCreg name | SUSMe005-A |
| Cite as: | SUSMe005-A (RRID:CVCL_D007) |
| Alternative name(s) |
HSF12
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator | Stanford University School of Medicine (SUSM) |
| Derivation country | United States |
External Databases |
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| Cellosaurus | CVCL_D007 |
| Wikidata | Q54896439 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| ZP removal technique | Mechanical |
| Cell isolation | Mechanical |
Culture Conditions
| Surface coating | Human lung fibroblasts | |||||||||
| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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