CHE-2

General

Cell Line

hPSCreg Name
SYSUe005-A
Alternative name(s)
CHE-2
Cell line type Human embryonic stem cell (hESC)
Last update 3rd September 2022
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Provider

Generator Sun Yat-sen University (SYSU)
Derivation country China

External Databases

Cellosaurus CVCL_C112
CLO CLO_0100475
Wikidata Q54971291

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics

How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
ZP removal technique Mechanical
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cells
Cellfinder Ont Id: EFO_0004040

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)