Generation of induced pluripotent stem cell line RCPCMi008-A derived from patient with spinocerebellar ataxia 17
Summary
IPSC line RCPCMi004-8 was generated from skin fibroblasts collected from a male patient with spinocerebellar ataxia 17. The patient has expanded trinucleotide CAG repeats in the TBP (TATA-binding protein) gene on chromosome 6q27. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4, and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and the formation of embryoid bodies. The RCPCMi008-A cell line carries the same trinucleotide CAG repeats in the TBP gene. The RCPCMi008-A cell line can be used to model Spinocerebellar ataxia in vitro. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Shuvalova LD, Davidenko AV, Eremeev AV, Khomyakova EA, Zerkalenkova EA, Lebedeva OS, Bogomazova AN, Klyushnikov SA, Illarioshkin SN, Lagarkova MA |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Jul;54:102431 |
| PubMed | 34171784 |
| DOI | 10.1016/j.scr.2021.102431 |