BTBGD-iPSC#2
KAIMRCi004-B
General
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Saudi |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
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| Family history | yes |
| Is the medical history available upon request? | yes |
| Is clinical information available? | yes |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA115112565 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | KING ABDULLAH INTERNATIONAL MEDICAL RESEARCH CENTER |
| Approval number | NRJ22J/005/01 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell line name |
erythroid progenitor cells (EPCs) Derived from same source line (potentially other lot and donor, see below):
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| Source cell type |
A unipotent hematopoietic progenitor cell derived from myeloid stem cells that is committed to the erythrocyte cell lineage.
Synonyms
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| Passage number reprogrammed | 1 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Notes on reprogramming vector detection | After passage 9 episcopal vectors were undetectable |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | Based on hESC-like morphology, emerging iPSCs were and picked and expanded into feeder-free culture conditions. |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Gelatin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| SOX17 |
Yes |
| GATA4 |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| CDX2 |
Yes |
| Brachyury or TBXT |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| NESTIN or NES |
Yes |
| PAX6 |
Yes |
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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