CIMHi44f028#4

General#

Cell Line

hPSCreg Name CIMHi001-A
Alternative name(s)
CIMHi44f028#4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th November 2018
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Provider

Generator Central Institute of Mental Health (CIMH)
Owner Department of Translational Brain Research
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA5230377

General Information

Is the cell line available in principle? Available with restrictions: Available for clinical and commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5230378

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
muscle of arm
A muscle organ that is part of an arm [Automatically generated definition].
Synonyms
  • muscle of upper arm or lower arm
  • arm skeletal muscle tissue
  • upper arm / lower arm muscle
  • arm skeletal muscle
  • arm muscle
show more synonyms
Age of donor (at collection) 40-44
Collected in 2013
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR
Notes on reprogramming vector detection absence of Sendai virus checked by RT-PCR after passage 8
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score
14.082 1.317
Report
pluritest_image01.png
Novelty score; Sample I corresponds to 028-line
pluritest_image02.png
pluripotency score; sample I corresponds to 028-line
Morphology pictures
Differentiation Potency
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vitro directed differentiation
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
In vitro directed differentiation
Morphology
028#4_d15_Map488+Tau555_20x_02.tif
Immunofluorescent analysis of axonal marker protein Tau and somatodendritic marker protein Map2a on d15 of terminal differentiation.

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)