Generation of three induced pluripotent stem cell lines carrying different variants of the BDNF (Val66Met) polymorphism


Brain-derived neurotrophic factor (BDNF) has been implicated in a multitude of neurodevelopmental processes including neuronal differentiation, axonal outgrowth, synaptic plasticity, or survival. One human-specific single nucleotide polymorphism (rs6265) in the BDNF gene causes a substitution of valine (Val) to methionine (Met) at codon 66 in the pro domain of the protein (Val66Met). This substitution is associated to reduced hippocampal volumes, poor performance on hippocampal-dependent memory tasks, and some mental disorders such as schizophrenia, depression or Alzheimer's disease. Here we generated three iPSC lines from healthy donors, either homozygous (Val/Val and Met/Met) or heterozygous (Val/Met) for the polymorphism. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Horschitz S, Frank J, Koch P
Journal Stem cell research
Publication Date 2023 Feb;66:102989
PubMed 36473250
DOI 10.1016/j.scr.2022.102989

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