NP0011-19

General#

Cell Line

hPSCreg Name UKKi009-B
Alternative name(s)
NP0011-19
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 3rd July 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA2825897
Cellosaurus CVCL_9S62
ECACC 66540032
EBiSC UKKi009-B

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 35-39
Ethnicity Caucasian, German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Prolonged QT interval
Long QT (LQT) Syndrome Type 2, mutation in KCNH2 gene c.3035_3045delTCCCTCGATGC, p.Leu1012Profs*55
The donor is affected.
Stage
Symptomatic
Synonyms
  • Long QT syndrome
  • Prolong qt interval on ekg
Genetic variants
KCNH2 (target)
7q36.1
1.NM_000238.3: c.3035_3045delTCCCTCGATGC
NP_000229.1:p.Leu1012Profs*55
Please explain briefly the supporting evidence
Disease associated phenotypes
  • prolonged QT interval on ECG
Family history Not known
Is the medical history available upon request? Yes
Is clinical information available? Medication available

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
HumanOmniExpressExome-8 BeadChip v1.2 (Illumina, Inc.)

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4584076

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of the Ruhr-University Bochum in Bad Oeynhausen
Approval number 41/2008
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of the Ruhr-University Bochum in Bad Oeynhausen
Approval number 41/2008
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 35-39
Collected in 2009
Passage number reprogrammed P3

Reprogramming method

Vector type Integrating
Vector Transposon (Sleeping beauty)
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones human ESC morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-80
Yes
Morphology pictures
Brightfield image of NP0011-19 iPSC colonies on day 2 post-thaw at high magnification; 32x objective
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
No
GSC
Yes
GATA6
Yes
CXCR4
Yes
PITX1
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
BMP4
Yes
GATA4
Yes
DCN
Yes
PECAM1 (CD31)
Yes
PDGF
No
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
Yes
B-TUBULIN
No
NEUROD1
Yes
HES5
Yes
FOXG1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 30

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: SNP-genotyping using HumanOmniExpressExome-8 BeadChip v1.2 (Illumina)

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes