HMGUi004-A
General
Cell Line |
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| hPSCreg name | HMGUi004-A |
| Cite as: | HMGUi004-A (RRID:CVCL_C9D6) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 18th June 2024 |
| User feedback | |
Provider |
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| Generator | Helmholtz Zentrum München (HMGU) |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA113476113 |
| Cellosaurus | CVCL_C9D6 |
| Wikidata | Q123031678 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46, XX
Karyotyping method:
G-Banding
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External Databases (Donor) |
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| BioSamples | SAMEA113476136 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethikkommission der Technischen Universität München |
| Approval number | Approval no. 2022-674-S-SR |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type | |
Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Medium |
mTeSR™ Plus
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
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| SSEA-1 |
Yes |
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Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| FOXA2 |
Yes |
| SOX17 |
Yes |
| CXCR4 |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| MESP1 |
Yes |
| TBXT |
Yes |
| MIXL |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| PAX6 |
Yes |
| SOX1 |
Yes |
| NCAD |
Yes |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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