VAL-9
ESe019-A
General
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics board of the Prince Felipe Research Centre |
| Approval number |
hESC Derivation
| Date of derivation | 2008-10-20 |
| Embryo stage | Morula |
| Supernumerary embryos from IVF treatment? |
Yes
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| PGD Embryo? |
No |
| ZP removal technique | Chemical |
| Cell isolation | None |
| Cell seeding | Isolated ICM |
iPSC Culture Conditions
| Surface coating | Gelatin | ||||||
| Feeder cells |
Human Foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
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| O2 Concentration | 21 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
VitroHES
Supplements
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iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| Amy |
No |
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| Cripto |
Yes |
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| GABRB3 |
Yes |
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| Nfh |
No |
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| Ren |
No |
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| Telomerase Activity |
Yes |
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Differentiation Potency
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
46XY
Passage number: 41
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Other Genotyping (iPSC Line) |
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