Generation of a lamin A/C knockout human induced pluripotent stem cell line (ZJULLi007-A) via CRISPR/Cas9
Summary
Lamin A/C is a protein encoded by the LMNA gene and belongs to the nuclear lamina protein family. Mutations in the LMNA gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. In this study, a lamin A/C knockout human induced pluripotent stem cell line was successfully generated using the CRISPR/Cas9 genome-editing technology, which was confirmed with normal pluripotency and karyotype. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Liu D, Shen J, Yang Z, Fan H, Wang H, Liang P, Gong T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Dec;81:103579 |
| PubMed | 39427442 |
| DOI | 10.1016/j.scr.2024.103579 |