NCL-7

General

Cell Line

hPSCreg name UNEWe007-A
Cite as:
UNEWe007-A (RRID:CVCL_A008)
Alternative name(s)
NCL-7
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 10th April 2017
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Provider

Generator University of Newcastle (UNEW)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A008
Wikidata Q54908235

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

Embryo stage Morula
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell seeding Embryo

Culture Conditions

Surface coating Gelatin
Feeder cells Swiss MF-1 Mice
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Medium conditioned by hES-NCL1 cells %
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TERT
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Passage number: 20

Other Genotyping (Cell Line)