Nott 2

General

Cell Line

hPSCreg name UNOTe002-A
Cite as:
UNOTe002-A (RRID:CVCL_A001)
Alternative name(s)
Nott 2
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 28th July 2017
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Provider

Generator University of Nottingham (UNOT)
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A001
Wikidata Q54930928

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation

Date of derivation 2005-05-17
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
glutamax
bFGF, LIF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
DNMT3B
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-1
No
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-3
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
24 of 30 cells=46XY. 1 cell=92XXYY, 1 cell=45Y-?X, 1 cell=42Y-1, 3 cells had rings and fragments.

Other Genotyping (Cell Line)