Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2
Summary
Induced pluripotent stem cell lines (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a ten years old boy with the type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2. PBMCs were reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and C-MYC. The iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry c.1814C>T(p.P605L) mutation in KCNH2 and have a normal karyotype. Thuse the iPSC line we established will be useful for studying the pathogenesis of the type 2 long QT syndrome and drug testing. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Wang T, Zhou Y, Zhou R, Huang W, Wang J, Li H, Lei M, Tan X, Zhang Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Oct;56:102509 |
| PubMed | 34438161 |
| DOI | 10.1016/j.scr.2021.102509 |