UKERi82A-S1-017

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
UKERi006-B
Alternative name(s)
UKERi82A-S1-017
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th April 2022
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Provider

Generator Universitätsklinikum Erlangen (UKER)
Owner Universitätsklinikum Erlangen (UKER)

External Databases

BioSamples SAMEA13024975
CLO CLO_0103746
Cellosaurus CVCL_YN18
Wikidata Q98133960

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104011170

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Ethics Committee Friedrich-Alexander University Erlangen-Nürnberg
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
fibroblast of dermis
Source cell origin
Skin of arm
Synonyms
  • Arm skin

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

CO2 Concentration 5 %
Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Differentiation Potency
Hematopoietic Precursor Cell
Ont Id: CL_0008001
In vitro directed differentiation
Marker Expressed
IBA1
Yes
PTPRC
Yes
SPN
Yes
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
DCX
Yes
SOX2
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 4
Karyotyping method: G-Banding

Other Genotyping (Cell Line)