Generation of a TSC2 knockout embryonic stem cell line by CRISPR/Cas9 editing
Summary
Tuberous Sclerosis Complex (TSC) is a severe developmental disorder with various clinical effects, primarily caused by TSC2 gene mutations, often involving loss of function(Henske,et al., 2016).To explore role of TSC2 in human heart development, we successfully developed a TSC2 knockout (TSC2-/-) human embryonic stem cells (hESCs) line using CRISPR/Cas9 gene editing. This TSC2-/- hESC line maintained a normal karyotype, expressed pluripotency markers strongly, and could differentiate into all three germ layers in vivo. This cell line will be a valuable tool for future research on the role of TSC2 in heart development. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Zhang S, Fan J, Sun H, Hao X, He Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103399 |
| PubMed | 38574666 |
| DOI | 10.1016/j.scr.2024.103399 |